Shadowless-illuminated variable-angle TIRF (siva-TIRF) microscopy for the observation of spatial-temporal dynamics in live cells
نویسندگان
چکیده
منابع مشابه
Spinning-Spot Shadowless TIRF Microscopy
Total internal reflection fluorescence (TIRF) microscopy is a powerful tool for visualizing near-membrane cellular structures and processes, including imaging of local Ca2+ transients with single-channel resolution. TIRF is most commonly implemented in epi-fluorescence mode, whereby laser excitation light is introduced at a spot near the periphery of the back focal plane of a high numerical ape...
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ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2014
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.5.001530